cdk8-specific monoclonal antibody  (Santa Cruz Biotechnology)

Journal: International journal of molecular sciences

Article Title: Limitations of Tamoxifen Application for In Vivo Genome Editing Using Cre/ER T2 System.

doi: 10.3390/ijms232214077

Figure Lengend Snippet: Figure 1. Detection of the tamoxifen-induced Cdk8 knockout in different organs of mice: (A) feeding method of tamoxifen administration; (B) injection method of tamoxifen administration. (C) Detection of the tamoxifen-induced Cdk8 knockout in the mouse brain and uterus (original oral and improved injection methods of tamoxifen administration).

Article Snippet: The membranes were blotted with primary anti CDK8 specific (sc-1521, SantaCruz, Santa Cruz, CA, USA) and anti β-actin specific antibodies (A2228, Sigma-Aldrich, Burlington, MA, USA) diluted in Tris buffered saline with Tween 20 (TBS-T; 1:1000) supplemented with 1% BSA overnight at 4 ◦C.

Techniques: Knock-Out, Injection

Journal: International journal of molecular sciences

Article Title: Limitations of Tamoxifen Application for In Vivo Genome Editing Using Cre/ER T2 System.

doi: 10.3390/ijms232214077

Figure Lengend Snippet: Figure 2. (A)—Schemes of loxP-sites localization in Cdk8 gene of Cdk8fl/fl mice (upper scheme) and Cdk8−/−mice (lower scheme) and annealing positions of PCR primers used for genotyping of animals.

Article Snippet: The membranes were blotted with primary anti CDK8 specific (sc-1521, SantaCruz, Santa Cruz, CA, USA) and anti β-actin specific antibodies (A2228, Sigma-Aldrich, Burlington, MA, USA) diluted in Tris buffered saline with Tween 20 (TBS-T; 1:1000) supplemented with 1% BSA overnight at 4 ◦C.

Techniques: